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Determination of all NOes in1H–13C–Me-ILV-U−2H–15N Proteins with Two Time-Shared Experiments

✍ Scribed by Dominique P. Frueh; David A. Vosburg; Christopher T. Walsh; Gerhard Wagner

Book ID
106401238
Publisher
Springer Netherlands
Year
2006
Tongue
English
Weight
442 KB
Volume
34
Category
Article
ISSN
0925-2738

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A 3D NOESY-(HCACO)NH experiment for the
A 3D NOESY-(HCACO)NH experiment for the measurement of NOEs involving1Hαin13C-/15N-labeled proteins dissolved in H2O
✍ Weixing Zhang; William H. Gmeiner 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 223 KB

A 3D NOESY-(HCACO)NH experiment is described that transfers NOEs from 1H alpha to the backbone 1Hn in the succeeding residue for detection. Using this strategy, NOEs involving 1H alpha protons that resonate exactly at the water frequency can be detected. NOEs from an overlapping 1H alpha proton that