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Potentiometric method for the determination of adenosine-5′-triphosphate

✍ Scribed by Takashi Katsu; Koji Yamanaka

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
324 KB
Volume
276
Category
Article
ISSN
0003-2670

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✦ Synopsis

A potentiometric method for the determhtation of adenosine4'4riphosphate (ATP) concentrations using a choline-sensitive membrane electrode and choline kinase enzyme is described. The choline electrode was constructed from a polyfvinyl chloridebbased membrane containing sodium tetraki~3,5-bis(2-methoxyhexaBuoro-2-propyl)phenyl]borate as an ion exchanger and 2-fluoro2'-nitrodiphenyl ether as a membrane solvent. Choline reacted quantitatively with ATP to produce the electrode-insensitive choline phosphate and, therefore, the concentration of ATP could be determined by the measurement of choline consumed by the enxymatic reaction. ATP down to 10 pM could be detected. This method was used successfully to determine the intracelhtlar ATP content of human red blood cells.

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