Graphene Functionalized Graphite Electrode with Diphenylacetylene for Sensitive Electrochemical Determination of Adenosine-5′-triphosphate
✍ Scribed by Chengxiang Ruan; Li Xu; Xiuzhen Wang; Jin Lou; Weimin Gao; Wei Sun
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 444 KB
- Volume
- 24
- Category
- Article
- ISSN
- 1040-0397
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✦ Synopsis
Abstract
In this paper a molecular wire modified carbon paste electrode (MW‐CPE) was firstly prepared by mixing graphite powder with diphenylacetylene (DPA). Then a graphene (GR) and chitosan (CTS) composite film was further modified on the surface of MW‐CPE to receive the graphene functionalized electrode (CTS‐GR/MW‐CPE), which was used for the sensitive electrochemical detection of adenosine‐5′‐triphosphate (ATP). The CTS‐GR/MW‐CPE exhibited excellent electrochemical performance and the electrochemical behavior of ATP on the CTS‐GR/MW‐CPE was carefully studied by cyclic voltammetry with an irreversible oxidation peak appearing at 1.369 V (vs. SCE). The electrochemical parameters such as charge transfer coefficient (α) and electrode reaction standard rate constant (k~s~) were calculated with the results of 0.53 and 5.28×10^−6^ s^−1^, respectively. By using differential pulse voltammetry (DPV) as detection technique, the oxidation peak current showed good linear relationship with ATP concentration in the range from 1.0 nM to 700.0 µM with a detection limit of 0.342 nM (3__σ__). The common coexisting substances, such as uric acid, ascorbic acid and guanosine‐5′‐triphosphate (GTP), showed no interferences and the modified electrode was successfully applied to injection sample detection.