✦ LIBER ✦
Determination of adenosine 5′-triphosphate by the luciferin-luciferase system with a stopped-flow spectrometer
✍ Scribed by S.G. Rhee; M.I. Greifner; P.B. Chock
- Publisher
- Elsevier Science
- Year
- 1975
- Tongue
- English
- Weight
- 299 KB
- Volume
- 66
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
A stopped-flow spectrometer is used for ATP assay by firefly luciferaseluciferin method. It allows one to record initial rise of the light intensity and to differentiate the light produced due to the conversion of ADP to ATP by nucleoside diphosphokinase in the firefly lantern when other nucleoside triphosphates are present. Addition of luciferin (0.27 mM) to luciferase extract increases the light intensity by a factor of 50-100. This method can be used to measure ATP in the picomole range.