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Potassium-induced reverse transformation of cells infected with a temperature-sensitive transformation mutant virus

✍ Scribed by Chiu-Nan Lai; Frederick F. Becker

Publisher
John Wiley and Sons
Year
1985
Tongue
English
Weight
471 KB
Volume
125
Category
Article
ISSN
0021-9541

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✦ Synopsis

High potassium concentrations altered the morphology and the ability to grow in soft agar in 6m2 cells, a clone of rat kidney cells infected with a temperature-sensitive mutant of Moloney sarcoma virus. Approximately 60% of cells exhibited normal morphology in the presence of 94.8 mM potassium in isotonic medium at the temperature permissive for transformation, whereas 100% were normal at 72 mM potassium in hypertonic media. A significant reduction of growth in soft agar was also induced with these conditions. However, the synthesis ratio of virus-specified transforming protein to marker viral protein was not altered. Na+K+-ATPase might play a role in this reversetransformation process.

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Abbreviations: ECM, extracellular matrix; SV40, simian virus 40; a-SMA, a-smooth cells were isolated from a single animal to develop homogeneous muscle actin; TGF-b1, transforming growth factor b1; PCR, polymerase chain reaction.