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Polymerase chain reaction for detecting Escherichia coli O157:H7

โœ Scribed by Jianghong Meng; Shaohua Zhao; Michael P. Doyle; Sharon E. Mitchell; Stephen Kresovich


Book ID
113275113
Publisher
Elsevier Science
Year
1996
Tongue
English
Weight
775 KB
Volume
32
Category
Article
ISSN
0168-1605

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t