Use of Real-Time Polymerase Chain Reacti
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Nathalie Y. Fortin; Ashok Mulchandani; Wilfred Chen
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Article
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2001
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Elsevier Science
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English
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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t