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An improved method for RNA extraction from carcass samples for detection of viable Escherichia coli O157:H7 by reverse-transcriptase polymerase chain reaction

✍ Scribed by S.C. De Wet; S.E. Denman; L. Sly; C.S. McSweeney


Book ID
111192200
Publisher
John Wiley and Sons
Year
2008
Tongue
English
Weight
181 KB
Volume
47
Category
Article
ISSN
0266-8254

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## Abstract A rapid, simple and efficient single‐tube procedure is described for the isolation of dengue virus RNA from small amount of serum (10 μl) followed by a reverse transcriptase‐polymerase chain reaction (RT‐PCR). Recovery of RNA is based on the lysing and nuclease‐inactivating properties o