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A Molecular Beacon-Based Duplex Real-Time Polymerase Chain Reaction Assay for Simultaneous Detection of Escherichia coli O157:H7 and Listeria monocytogenes in Milk and Milk Products

โœ Scribed by Singh, Jitender; Batish, Virender K.; Grover, Sunita


Book ID
118236880
Publisher
Mary Ann Liebert
Year
2009
Tongue
English
Weight
140 KB
Volume
6
Category
Article
ISSN
1535-3141

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Molecular beacons (MBs) are oligonucleotide probes that fluoresce upon hybridization. In this paper, we described the development of a real-time PCR assay to detect the presence of Escherichia coli O157:H7 using these fluorogenic reporter molecules. MBs were designed to recognize a 26-bp region of t

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