Polyamines prevent apoptotic cell death in cultured cerebellar granule neurons
β Scribed by Jun Harada; Masahiko Sugimoto
- Book ID
- 114002418
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 600 KB
- Volume
- 753
- Category
- Article
- ISSN
- 0006-8993
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## Abstract Cultured cerebellar granule neurons (CGNs) undergo apoptosis when deprived of depolarizing stimulation and provide an in vitro model system with which to study the effects of neurotrophic substances. Our previous results showed that secretory phospholipases A~2~ (sPLA~2~s) protect CGNs
When cultured cerebellar granule neurons (CGN) are transferred from 25 mM KCl (K25) to 5 mM KCl (K5) caspase-3 and caspase-8, but not caspase-1 or caspase-9,activities are induced and cells die apoptotically. CGN death was triggered by a [Ca(2+)](i) modification when [Ca(2+)](i) was reduced from 300