Poly(3-hydroxybutyrate) production from xylose by recombinant Escherichia coli
โ Scribed by Sang Yup Lee
- Publisher
- Springer
- Year
- 1998
- Tongue
- English
- Weight
- 93 KB
- Volume
- 18
- Category
- Article
- ISSN
- 1615-7605
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Several recombinant Escherichia coli strains, including XLI-Blue, JM109, HB101, and DH5a harboring a stable high-copynumber plasmid pSYL105 containing the Alcaligenes eutrophus polyhydroxyal ka noate (PHA) biosynt hesis genes were constructed. These recombinant strains were examined for their abilit
A simple method for the recovery of microbial poly(3-hydroxybutyrate) [P(3HB)] from recombinant Escherichia coli harboring the Ralstonia eutropha PHA biosynthesis genes was developed. Various acids (HCl, H 2 SO 4 ), alkalies (NaOH, KOH, and NH 4 OH), and surfactants (dioctylsulfosuccinate sodium sal