High throughput study of separation of poly(3-hydroxybutyrate) from recombinant Escherichia coli XL1 blue

Efficient and economical recovery of pol

Efficient and economical recovery of poly(3-hydroxybutyrate) from recombinant Escherichia coli by simple digestion with chemicals

✍ Jong-il Choi; Sang Yup Lee 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 223 KB 👁 2 views

A simple method for the recovery of microbial poly(3-hydroxybutyrate) [P(3HB)] from recombinant Escherichia coli harboring the Ralstonia eutropha PHA biosynthesis genes was developed. Various acids (HCl, H 2 SO 4 ), alkalies (NaOH, KOH, and NH 4 OH), and surfactants (dioctylsulfosuccinate sodium sal