Metabolic and kinetic analysis of poly(3-hydroxybutyrate) production by recombinant Escherichia coli
β Scribed by Richard J. van Wegen; Sang-Yup Lee; Anton P. J. Middelberg
- Publisher
- John Wiley and Sons
- Year
- 2001
- Tongue
- English
- Weight
- 228 KB
- Volume
- 74
- Category
- Article
- ISSN
- 0006-3592
- DOI
- 10.1002/bit.1096
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A simple method for the recovery of microbial poly(3-hydroxybutyrate) [P(3HB)] from recombinant Escherichia coli harboring the Ralstonia eutropha PHA biosynthesis genes was developed. Various acids (HCl, H 2 SO 4 ), alkalies (NaOH, KOH, and NH 4 OH), and surfactants (dioctylsulfosuccinate sodium sal
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## Abstract The metabolic burden and the stress load resulting from temperatureβinduced production of human basic fibroblast growth factor is connected to an increase in the respiratory activity of recombinant __Escherichia coli__, thereby reducing the biomass yield. To study the underlying changes