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Plant regeneration from callus culture of threeCitrusrootstocks

✍ Scribed by Naïma Beloualy

Publisher
Springer
Year
1991
Tongue
English
Weight
951 KB
Volume
24
Category
Article
ISSN
0167-6857

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✦ Synopsis

Complete plants were regenerated from callus derived embryo culture of Citrus aurantium, Poncirus trifoliata and of Carrizo citrange (C. sinensis × Poncirus trifoliata) via somatic embryogenesis and organogenesis. Isolated embryoids were differentiated on medium containing 1000 to 1500mgl -~ malt extract. Both embryoids and shoots were obtained by the use of plant growth regulators: NAA and BAP. The highest frequency was obtained with I mgl ~ NAA + 5 mg 1-~ BAP in Poncirus trifoliata and citrange but with 1 mgl -~ NAA + 10mgl -~ BAP in C. aurantium. Rooting was promoted by a supplement of 1 mgl ~ GA 3 or 1 mgl ~ NAA. GA 3 enhanced stem elongation and rooting in embryoids and NAA stimulated adventitious root formation.

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