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Plant regeneration from callus culture in potato

✍ Scribed by B. S. Ahloowalia

Publisher: Springer
Year: 1982
Tongue: English
Weight: 353 KB
Volume: 31
Category: Article
ISSN: 0014-2336
DOI: 10.1007/bf00039214

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✦ Synopsis

A procedure for plant regeneration from callus culture of potato, Solunum tuberosum L. is described. Calli were induced from l-2 mm long shoot apices of potato cultivars Cara and A25/19 on half-strength Murashige and Skoog's medium (half-MS) supplemented with 3.2 mg IAA (indole-3-acetic acid), 1.0 mg kinetin (6-furfurylamino)purine], and 0.5 mg 2,4-D [2,4dichlorophenoxy)acetic acid]/l. Sixty percent explants produced nodular calli on this medium within 30 days. Calli differentiated into shoot-primordia when subcultured on half-MS medium supplemented with 0.5 mg 2,4-D and 1 .O mg zeatin [6-(4-hydroxy-3-methybut-2 enylamino)amino purine]/l. Differentiated calli on half-MS medium without growth hormones produced complete plantlets which were cloned on the same medium and transferred into soil.

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