𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Plant regeneration from callus cultures ofPiper longumL. by organogenesis

✍ Scribed by S. R. Bhat; A. Kackar; K. P. S. Chandel

Publisher
Springer
Year
1992
Tongue
English
Weight
520 KB
Volume
11
Category
Article
ISSN
0721-7714

No coin nor oath required. For personal study only.

✦ Synopsis

Plant regeneration from callus cultures of Piper longum was achieved through organogenesis. In vitro grown shoots were used as explants for callus induction. Competent callus was initiated around the nodal ring of tissue using Murashige and Skoog medium supplemented with 1.0 mg.l(-1)Ξ±- naphthaleneacetic acid and 0.2 mg.l(-1) N(6)-benzyladenine. Optimum growth regulator concentrations for shoot induction and shoot elongation were found to be 0.5 mg.l(-1) indole-3-acetic acid with 1.5 mg.l(-1) benzyladenine, and 0.1 mg.l(-1) indole-3-acetic acid with 0.2 mg.l(-1) benzyladenine, respectively. Elongated shoots were rooted on half-strength Murashige and Skoog medium having 0.1 mg.l(-1) indole3-acetic acid. The rooted plants were successfully established in soil.

πŸ“œ SIMILAR VOLUMES

Whole plant regeneration ofCicer arietin
Whole plant regeneration ofCicer arietinumfrom callus cultures via organogenesis
✍ K. S. Barna; A. K. Wakhlu πŸ“‚ Article πŸ“… 1994 πŸ› Springer 🌐 English βš– 885 KB

A protocol for whole plant regeneration of Cicer arietinum L. cv. C-235 via organogenesis from callus has been developed. Callus initiation was best when immature leaflets were cultured on MS medium containing 5 or 25 ΞΌM 2,4-D or NAA in combination with 10 ΞΌM BA, or 25 ΞΌM 2,4-D alone. The callus gre

Plant regeneration from callus culture i
Plant regeneration from callus culture in potato
✍ B. S. Ahloowalia πŸ“‚ Article πŸ“… 1982 πŸ› Springer 🌐 English βš– 353 KB

A procedure for plant regeneration from callus culture of potato, Solunum tuberosum L. is described. Calli were induced from l-2 mm long shoot apices of potato cultivars Cara and A25/19 on half-strength Murashige and Skoog's medium (half-MS) supplemented with 3.2 mg IAA (indole-3-acetic acid), 1.0 m

Plant regeneration from callus culture o
Plant regeneration from callus culture of threeCitrusrootstocks
✍ NaΓ―ma Beloualy πŸ“‚ Article πŸ“… 1991 πŸ› Springer 🌐 English βš– 951 KB

Complete plants were regenerated from callus derived embryo culture of Citrus aurantium, Poncirus trifoliata and of Carrizo citrange (C. sinensis Γ— Poncirus trifoliata) via somatic embryogenesis and organogenesis. Isolated embryoids were differentiated on medium containing 1000 to 1500mgl -~ malt ex