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Physiological and biochemical characterization of intergeneric hybrids of thermotolerant and non-thermotolerant yeasts

โœ Scribed by Dr. S. S. Dhamija; R. Gera; D. K. Singh; D. Singh; R. Marchant

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
364 KB
Volume
37
Category
Article
ISSN
0233-111X

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โœฆ Synopsis

Kluyveromyces-like intergeneric hybrids of thermotolerant Kluyveromyces marxianus and non-thermotolerant Saccharomyces cerevisiae, produced in a previous study by protoplasmic fusion, have been characterized. On molasses, these strains produced ethanol in excess of 6% (v/v) both at 30 and 45 "C as against 3% and 4.2% (v/v) by the former parent at 30 and 45 "C, respectively. In hybrids, the increase in ethanol production appeared to be a sequel to increased activities of alcohol dehydrogenase and pyruvate kinase, derived probably from S. cerevisiae parent. Hybrid ADH-isozyme pattern on polyacrylamide gel corroborated the presence of S. cerevisiae ADH in the tested hybrids. Regression analyses indicated a positive correlation between ethanol production and ADH or PK or both ( r -0.76-0.84).

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