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Phosphorylation of surface E-selectin and the effect of soluble ligand (Sialyl Lewisx) on the half-life of E-selectin

✍ Scribed by Edgar F. Smeets; Theodora De Vries; Jet F. M. Leeuwenberg; Dirk H. van den Eijnden; Wim A. Buurman; Jacques J. Neefjes

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
638 KB
Volume
23
Category
Article
ISSN
0014-2980

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✦ Synopsis

Phosphorylation of surface E-selectin and the effect of soluble ligand (Sialyl Lewis") on the half-life of E-selectin* E-selectin (ELAM-1) is an adhesion molecule for leukocytes that is transiently expressed on endothelial cells. Following cell surface expression of E-selectin on human umbilical vein endothelial cells (HUVEC) stimulated with tumor necrosis factor, the induced E-selectin molecules are rapidly degraded. The kinetics of turnover of surface disposed E-selectin were investigated. The rapid disappearance of surface E-selectin is temperature dependent and sensitive to the lysosomotropic agent chloroquine. The half-life of E-selectin is not affected by inclusion of soluble sialyl Lewis x (sLeX) ligands in the medium. Surface E-selectin is phosphorylated on one or more serine residues, but this modification is not obviously related to internalization.

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## Abstract ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract, please click on HTML or PDF.

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The expression of E-selectin receptors on the surface of endothelial cells stimulated by cytokines i s one of the first steps in inflammatory processes.[" E-selectin i s a membrane-resident ylycoprotein belonging to the selectin family (E-selectin. L-selectin, P-selectin) which specifically binds th