I4C-Labeled inosine 5'-phosphate was synthesized by direct phosphorylation of i n 0 s i n e -8 -~~C with phosphoryl chloride followed by hydrolysis of the resulting phosphorodichloridate. Isolation and recrystallization o f its disodium salt afforded pure material in 66.8% overall chemical yield.
Phosphate Esters of Human Erythrocytes: Synthesis of ITP-14C from Inosine-8-14C
β Scribed by VANDERHEIDEN, B. S.
- Book ID
- 109659767
- Publisher
- Nature Publishing Group
- Year
- 1967
- Tongue
- English
- Weight
- 411 KB
- Volume
- 216
- Category
- Article
- ISSN
- 0028-0836
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π SIMILAR VOLUMES
A new enzymatic method for the synthesis of ['4C]pyridoxal 5'-phosphate is presented. [ r4C]Pyridoxal 5'-phosphate was synthesized from [ "C]pyridoxine through the successive actions of pyridoxal kinase and pyridoxamine 5'-phosphate oxidase in a reaction mixture containing ATP, ['4C]pyridoxine. and
All solvents and reagents were purified and/or dried before using according to generally accepted procedures, unless otherwise stated. I4CH3I was purchased from New England Nucleor Corp. Prep. HPLC: Micromeritics liquid chromatograph, refractive-index detector. Anal. TLC: F254 TLC plates (Merck). Pr