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Phage display of enzymes and in vitro selection for catalytic activity

✍ Scribed by Patrice Soumillion; Laurent Jespers; Mich’ele Bouchet; Jacqueline Marchand-Brynaert; Pascale Sartiaux; Jacques Fastrez

Book ID
112893870
Publisher
Springer-Verlag
Year
1994
Tongue
English
Weight
995 KB
Volume
47
Category
Article
ISSN
0273-2289

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✍ Isabelle Ponsard; Moreno Galleni; Patrice Soumillion; Jacques Fastrez 📂 Article 📅 2001 🏛 John Wiley and Sons 🌐 English ⚖ 116 KB 👁 1 views

The metallo-b-lactamase bLII from Bacillus cereus 569/H/9 was displayed on the filamentous phage fd. The phage-bound enzyme fd-bLII was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(II ) ion with EDTA and reactivated by addition of