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Selection of catalytically active biotin ligase and trypsin mutants by phage display

✍ Scribed by Heinis, C.; Huber, A.; Demartis, S.; Bertschinger, J.; Melkko, S.; Lozzi, L.; Neri, P.; Neri, D.


Book ID
118160983
Publisher
Oxford University Press
Year
2001
Tongue
English
Weight
376 KB
Volume
14
Category
Article
ISSN
1741-0126

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The metallo-b-lactamase bLII from Bacillus cereus 569/H/9 was displayed on the filamentous phage fd. The phage-bound enzyme fd-bLII was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(II ) ion with EDTA and reactivated by addition of