The metallo-b-lactamase bLII from Bacillus cereus 569/H/9 was displayed on the filamentous phage fd. The phage-bound enzyme fd-bLII was shown to be active on benzylpenicillin as substrate; it could be inactivated by complexation of the essential zinc(II ) ion with EDTA and reactivated by addition of
A Method for the Selection of Catalytic Activity Using Phage Display and Proximity Coupling
β Scribed by Jean-Luc Jestin; Peter Kristensen; Greg Winter
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 108 KB
- Volume
- 38
- Category
- Article
- ISSN
- 0044-8249
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