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A strategy for the isolation of catalytic activities from repertoires of enzymes displayed on phage

✍ Scribed by Salvatore Demartis; Adrian Huber; Francesca Viti; Luisa Lozzi; Leonardo Giovannoni; Paolo Neri; Greg Winter; Dario Neri

Book ID
115629065
Publisher
Elsevier Science
Year
1999
Tongue
English
Weight
434 KB
Volume
286
Category
Article
ISSN
0022-2836

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A mixture of phages displaying the wild type and four mutant Rtem 13-1actamases was submitted to three rounds of selection by incubation with a biotinylated suicide inhibitor followed by chromatography on streptavidin coated beads. The final mixture of selected phages was shown to be made up of two