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Percoll density gradient centrifugation

โœ Scribed by Donato Fumarola; Salvatore Antonaci; Emilio Jirillo; Irene Munno; Giacomo Lucivero; Lorenzo Bonomo


Book ID
105652295
Publisher
Springer
Year
1982
Tongue
English
Weight
348 KB
Volume
12
Category
Article
ISSN
0940-5437

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## Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory