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Rapid isolation of muscle-derived stem cells by discontinuous Percoll density gradient centrifugation

โœ Scribed by Xiaoxia Che; Jie Guo; Bangkang Wang; Yuxing Bai

Book ID
107427378
Publisher
Society for In Vitro Biology
Year
2011
Tongue
English
Weight
219 KB
Volume
47
Category
Article
ISSN
1475-2689

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## Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory