Isolation of mitochondria from rat brain using Percoll density gradient centrifugation
โ Scribed by Sims, Neil R; Anderson, Michelle F
- Book ID
- 109946480
- Publisher
- Nature Publishing Group
- Year
- 2008
- Tongue
- English
- Weight
- 259 KB
- Volume
- 3
- Category
- Article
- ISSN
- 1750-2799
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๐ SIMILAR VOLUMES
## Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory
A method for preparing suspensions of hepatocytes with long-term viability is described. Its originality lies in the use, after conventional preparation, of a Percoll density gradient centrifugation which allows a complete and rapid separation of cell debris and released proteases from the intact he