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Peptides modified by myristoylation activate eNOS in endothelial cells through Akt phosphorylation

โœ Scribed by Karina Krotova; Hanbo Hu; Shen-Ling Xia; Leonid Belayev; Jawaharlal M Patel; Edward R Block; Sergey Zharikov


Book ID
110009166
Publisher
Nature Publishing Group
Year
2006
Tongue
English
Weight
233 KB
Volume
148
Category
Article
ISSN
0007-1188

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med