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Activation of nitric oxide synthase in endothelial cells by Akt-dependent phosphorylation

✍ Scribed by Zeiher, Andreas M.; Dimmeler, Stefanie; Fleming, Ingrid; Fisslthaler, Beate; Hermann, Corinna; Busse, Rudi


Book ID
109653859
Publisher
Nature Publishing Group
Year
1999
Tongue
English
Weight
276 KB
Volume
399
Category
Article
ISSN
0028-0836

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Activation of nitric oxide synthase in e
✍ Zeiher, Andreas M.; Dimmeler, Stefanie; Fleming, Ingrid; Fisslthaler, Beate; Her πŸ“‚ Article πŸ“… 1999 πŸ› Nature Publishing Group 🌐 English βš– 276 KB

1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med