Activation of nitric oxide synthase in e
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Zeiher, Andreas M.; Dimmeler, Stefanie; Fleming, Ingrid; Fisslthaler, Beate; Her
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Article
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1999
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Nature Publishing Group
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English
β 276 KB
1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med