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Serine 1179 phosphorylation of endothelial nitric oxide synthase caused by 2,4,6-trinitrotoluene through PI3K/Akt signaling in endothelial cells

โœ Scribed by Yang Sun; Daigo Sumi; Yoshito Kumagai


Book ID
113923862
Publisher
Elsevier Science
Year
2006
Tongue
English
Weight
487 KB
Volume
214
Category
Article
ISSN
0041-008X

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1179A eNOS and HA-Akt overnight. 36 h after transfection, we placed cells into dialysed serum-replete, phosphate-free Dulbecco's minimum essential medium supplemented with 80 Ci per ml of 32 P orthophosphoric acid for 3 h. Some cells were pretreated with wortmannin (500 nM) in the phosphate-free med