Peptide mapping of proteins from acrylamide gels
β Scribed by Dennis Bray; Susan M. Brownlee
- Book ID
- 107711450
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 484 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0003-2697
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Residual acrylamide can cause severe suppression of signal intensity during matrix-assisted laser desorption/ionization (MALDI) peptide mass mapping experiments. This suppression phenomenon can compromise the ability to detect low picomole and subpicomolar amounts of peptides extracted from twodimen
Proteins in polyacrylamide gels can be rapidly visualized by soaking in trichloroacetic acid or chloroform followed by illumination with UV light. The UV-lightdriven reaction of tryptophan in the presence of trichlorocompounds yields products that emit sufficiently in the visible region to identify