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Visualization of Proteins in Acrylamide Gels Using Ultraviolet Illumination

✍ Scribed by Dmitri Kazmin; Robert A. Edwards; Raymond J. Turner; Eric Larson; Jean Starkey

Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
173 KB
Volume
301
Category
Article
ISSN
0003-2697

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✦ Synopsis

Proteins in polyacrylamide gels can be rapidly visualized by soaking in trichloroacetic acid or chloroform followed by illumination with UV light. The UV-lightdriven reaction of tryptophan in the presence of trichlorocompounds yields products that emit sufficiently in the visible region to identify the location of the protein bands on the gel. This method can be used to rapidly identify protein bands on a gel in less than 20 min. On thin polyacrylamide gels, 1.0 g of protein can easily be detected for proteins with typical tryptophan percentages.

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