✦ LIBER ✦

Effect of residual acrylamide monomer from two-dimensional gels on matrix-assisted laser desorption/ionization peptide mass mapping experiments

✍ Scribed by Erwin J. Alvarez; Barbara S. Larsen; Christopher D. Coldren; Janet Rice

Book ID: 101239612
Publisher: John Wiley and Sons
Year: 2000
Tongue: English
Weight: 46 KB
Volume: 14
Category: Article
ISSN: 0951-4198
DOI: 10.1002/(sici)1097-0231(20000615)14:11<974::aid-rcm974>3.0.co;2-h

No coin nor oath required. For personal study only.

✦ Synopsis

Residual acrylamide can cause severe suppression of signal intensity during matrix-assisted laser desorption/ionization (MALDI) peptide mass mapping experiments. This suppression phenomenon can compromise the ability to detect low picomole and subpicomolar amounts of peptides extracted from twodimensional gels. A rapid and simple method that exploits the use of pipette tips incorporating C18 packing materials for the enhancement of MALDI signal intensity is presented. The utility of the method is demonstrated with peptide solutions incorporating residual acrylamide and/or gel monomer components.

📜 SIMILAR VOLUMES

Peptide mapping of proteins in cerebrosp

Peptide mapping of proteins in cerebrospinal fluid utilizing a rapid preparative two-dimensional electrophoretic procedure and matrix-assisted laser desorption/ionization mass spectrometry

✍ Pia Davidsson; Carol L. Nilsson 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 274 KB

Peptide mass fingerprint sequence covera

Peptide mass fingerprint sequence coverage from differently stained proteins on two-dimensional electrophoresis patterns by matrix assisted laser desorption/ionization-mass spectrometry (MALDI-MS)

✍ Christian Scheler; Stephanie Lamer; Zaoming Pan; Xin-Ping Li; Johannes Salnikow; 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 939 KB

Protein Identification on Two-Dimensiona

Protein Identification on Two-Dimensional Gels Archived Nearly Two Decades Ago by In-Gel Digestion and Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry

✍ Hiroyuki Matsumoto; Naoka Komori 📂 Article 📅 1999 🏛 Elsevier Science 🌐 English ⚖ 78 KB

Impact of deglycosylation methods on two

Impact of deglycosylation methods on two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight-mass spectrometry for proteomic analysis

✍ Beth G. Fryksdale; Paul T. Jedrzejewski; David L. Wong; Alfred L. Gaertner; Bria 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 282 KB 👁 2 views

Impact of prefractionation using Gradifl

Impact of prefractionation using Gradiflow™ on two-dimensional gel electrophoresis and protein identification by matrix assisted laser desorption/ionization-time of flight-mass spectrometry

✍ Lisa Pang; Beth G. Fryksdale; Nicole Chow; David L. Wong; Alfred L. Gaertner; Br 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 283 KB 👁 1 views

Fast visible dye staining of proteins in

Fast visible dye staining of proteins in one- and two-dimensional sodium dodecyl sulfate-polyacrylamide gels compatible with matrix assisted laser desorption/ionization-mass spectrometry

✍ Jung-Kap Choi; Ho-Zoon Chae; Sun-Young Hwang; Hoon-In Choi; Li-Tai Jin; Gyurng-S 📂 Article 📅 2004 🏛 John Wiley and Sons 🌐 English ⚖ 124 KB