Histidine decarboxylase (EC 4.1.1.22) prepared from a murine mastocytoma is activated up to six-fold when the concentration of phosphate in the assay medium is increased from 1 mM to 150 mM. Chloride and sulfate, on the other hand, are inhibitory and appear to interfere with the binding of pyridoxal
Peptide inhibition of mammalian histidine decarboxylase
✍ Scribed by Lena Hammar; Ulf Ragnarsson
- Publisher
- SP Birkhäuser Verlag Basel
- Year
- 1979
- Tongue
- English
- Weight
- 407 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1420-908X
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This report describes a microassay procedure for mammalian histidine decarboxylase (HDC) based on the measurement of ['YZ]O, formed from L-[l-Wlhistidine. This assay is particularly useful for quick measurement of HDC activity both in microgram quantities of cell or tissue extract and in tissues th
Histamine is a multifunctional biogenic amine with relevant roles in intercellular communication, inflammatory processes and highly prevalent pathologies. Histamine biosynthesis depends on a single decarboxylation step, carried out by a PLP-dependent histidine decarboxylase activity (EC 4.1.1.22), a