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A microassay for mammalian histidine decarboxylase

✍ Scribed by David G. Ritchie; David A. Levy

Publisher: Elsevier Science
Year: 1975
Tongue: English
Weight: 690 KB
Volume: 66
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(75)90737-x

No coin nor oath required. For personal study only.

✦ Synopsis

This report describes a microassay procedure for mammalian histidine decarboxylase (HDC) based on the measurement of ['YZ]O, formed from L-[l-Wlhistidine.

This assay is particularly useful for quick measurement of HDC activity both in microgram quantities of cell or tissue extract and in tissues that contain significant levels of endogenous histamine.

Using this assay, we have shown that the pH optimum, K, and thermolability of HDC are similar for extracts prepared both from normal rat peritoneal mast cells and from the Furth mouse mastocytoma. HDC activity could be detected in homogenates prepared from IO5 rat mast cells, and it was expressed on a per cell basis. Mast cell HDC activity varied with the strain of rat from which the cells were obtained and with the season when they were assayed.

' In partial fulfillment of the requirements of Johns Hopkins University for the degree of Doctor of Philosophy.

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