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PARTIAL PURIFICATION AND CHARACTERIZATION OF SWEET CORN GERM LIPOXYGENASE

โœ Scribed by CHOCKCHAI THEERAKULKAIT; DIANE M. BARRETT


Book ID
111337015
Publisher
John Wiley and Sons
Year
1994
Tongue
English
Weight
871 KB
Volume
18
Category
Article
ISSN
0145-8884

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Partial purification and some properties
โœ B. Bisakowski; S. Kermasha; C. Schuepp ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Springer ๐ŸŒ English โš– 236 KB

A partially purified lipoxygenase extract was obtained from the yeast Saccharomyces cerevisiae by precipitation with solid (NH4)SO4 at 20% to 80% saturation. The enzyme had two pH optima, at pH 8.0 and 10.0, with respective apparent K m values of 13 and 9.5 ฮผM. At both pH optima, the lipoxygenase de