## Abstract The 38βkDa lipoprotein is one of the most potent cell surface immunogens of __Mycobacterium tuberculosis__ in antibodyβand T cellβmediated reactions. Using a pure recombinant form of the protein, we have recently shown that it binds phosphate much like that of the phosphateβbinding prot
Overexpression, crystallization and preliminary X-ray crystallographic analysis of the phosphotriesterase from Mycobacterium tuberculosis
β Scribed by Zhang, Liang; Chen, Ruyi; Dong, Zhe; Li, Xin
- Book ID
- 119771660
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 285 KB
- Volume
- 69
- Category
- Article
- ISSN
- 1744-3091
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Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53
## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz