Overexpression, crystallization and preliminary X-ray crystallographic analysis of pyridoxal biosynthesis lyase PdxS from Pyrococcus horikoshii
โ Scribed by Yoon, Ji Young ;Park, Chan Ryang ;Lee, Hyung Ho ;Suh, Se Won
- Book ID
- 114529359
- Publisher
- John Wiley and Sons
- Year
- 2012
- Tongue
- English
- Weight
- 221 KB
- Volume
- 68
- Category
- Article
- ISSN
- 1744-3091
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Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53
## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz