Crystallization and Preliminary X-Ray Crystallographic Analysis of Carboxylesterase from Pseudomonas fluorescens
โ Scribed by K.K. Kim; K.Y. Hwang; W.H. Jang; H.H. Lee; O.J. Yoo; M.U. Choi; S.W. Suh
- Book ID
- 115564425
- Publisher
- Elsevier Science
- Year
- 1993
- Tongue
- English
- Weight
- 311 KB
- Volume
- 302
- Category
- Article
- ISSN
- 0003-9861
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Single crystals of arylesterase (EC 3.1.1.2) from Vibrio mimicus have been obtained from ammonium sulfate as a precipitant at room temperature for 2 months. The present crystals diffract up to 2.2 A resolution and belong to monoclinic space group P2(1). The cell dimensions are a = 55.65(1) A, b = 53
## Abstract A recombinant form of __Rhodobacter sphaeroides__ phosphoribulokinase (PRK), expressed in __Escherichia coli__ and isolated by affinity chromatography, was crystallized by the sitting drop vapor diffusion technique using NH~4~H~2~PO~4~ (pH 5.6) as the precipitating agent. PRK crystalliz