✦ LIBER ✦

Optimizing protein extraction from plant tissues for enhanced proteomics analysis

✍ Scribed by Wei Wang; Fuju Tai; Shaoning Chen

Publisher: John Wiley and Sons
Year: 2008
Tongue: English
Weight: 570 KB
Volume: 31
Category: Article
ISSN: 1615-9306
DOI: 10.1002/jssc.200800087

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✦ Synopsis

Abstract

Plant tissues usually contain high levels of proteases and secondary metabolites that severely interfere with protein extraction, separation, and identification. Preparation of high‐quality protein samples from plant tissues for proteomic analysis represents a great challenge. This article briefly describes the critical points in protein separation, especially secondary metabolites in plant tissues, and removal strategy. It provides an updated overview of three total protein extraction methods and their applications in proteomic analysis of various recalcitrant tissues.

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