✦ LIBER ✦

A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis

✍ Scribed by Wei Wang; Rita Vignani; Monica Scali; Mauro Cresti

Publisher: John Wiley and Sons
Year: 2006
Tongue: English
Weight: 779 KB
Volume: 27
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200500722

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✦ Synopsis

Abstract

A simple and universally applicable protocol for extracting high‐quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separation of proteins. As the protocol is rapid, universal, and compatible with silver staining, it could be used for routine protein extraction from recalcitrant plant tissues for proteomic analysis.

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