✦ LIBER ✦

Extraction of proteins from plant tissues for two-dimensional electrophoresis analysis

✍ Scribed by Patrick Giavalisco; Eckhard Nordhoff; Hans Lehrach; Johan Gobom; Joachim Klose

Publisher: John Wiley and Sons
Year: 2003
Tongue: English
Weight: 911 KB
Volume: 24
Category: Article
ISSN: 0173-0835
DOI: 10.1002/elps.200390016

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

Extraction of plant proteins for two-dim

Extraction of plant proteins for two-dimensional electrophoresis

✍ Dr. Fabienne Granier 📂 Article 📅 1988 🏛 John Wiley and Sons 🌐 English ⚖ 684 KB

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membr

Protein extraction for two-dimensional e

Protein extraction for two-dimensional electrophoresis from olive leaf, a plant tissue containing high levels of interfering compounds

✍ Wei Wang; Monica Scali; Rita Vignani; Antonia Spadafora; Elisabetta Sensi; Silvi 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 275 KB

Protein extraction from Phoenix dactylif

Protein extraction from Phoenix dactylifera L. leaves, a recalcitrant material, for two-dimensional electrophoresis

✍ Sonia Gómez-Vidal; Manuel Tena; Luis Vicente Lopez-Llorca; Jesús Salinas 📂 Article 📅 2008 🏛 John Wiley and Sons 🌐 English ⚖ 448 KB

## Abstract This work was aimed at optimizing a protein extraction procedure for date palm (__Phoenix dactylifera__ L.) leaves, a highly recalcitrant plant tissue for 2‐DE. Five protein extraction protocols based on different protein precipitation agents (TCA/acetone __vs.__ phenol (Ph) methods) an

A universal and rapid protocol for prote

A universal and rapid protocol for protein extraction from recalcitrant plant tissues for proteomic analysis

✍ Wei Wang; Rita Vignani; Monica Scali; Mauro Cresti 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 779 KB

## Abstract A simple and universally applicable protocol for extracting high‐quality proteins from recalcitrant plant tissues is described. We have used the protocol with no modification, for a wide range of leaves and fruits. In all cases, this protocol allows to obtain good electrophoretic separa

Analysis of human tear proteins by two-d

Analysis of human tear proteins by two-dimensional electrophoresis

✍ Dr. Seijiro Mii; Kazuyuki Nakamura; Kazusuke Takeo; Shinji Kurimoto 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 456 KB 👁 1 views

Analysis of Protein–Protein Interaction

Analysis of Protein–Protein Interaction by Two-Dimensional Affinity Electrophoresis

✍ Isamu Kameshita; Atsuhiko Ishida; Hitoshi Fujisawa 📂 Article 📅 1998 🏛 Elsevier Science 🌐 English ⚖ 159 KB