Extraction of plant proteins for two-dimensional electrophoresis

✍ Scribed by Dr. Fabienne Granier

• Publisher: John Wiley and Sons
• Year: 1988
• Tongue: English
• Weight: 684 KB
• Volume: 9
• Category: Article
• ISSN: 0173-0835
• DOI: 10.1002/elps.1150091106

✦ Synopsis

Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membrane proteins in addition to a recovery of soluble proteins, and (iii) a trichloroacetic acid-acetone procedure allowing the direct precipitation of total proteins.