Advances in protein solubilisation for two-dimensional electrophoresis
โ Scribed by Ben Herbert
- Publisher
- John Wiley and Sons
- Year
- 1999
- Tongue
- English
- Weight
- 67 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0173-0835
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Three different extraction procedures for two-dimensional electrophoresis of plant proteins are compared: (i) extraction of soluble proteins with a nondenaturing Tris-buffer, (ii) denaturing extraction in presence of sodium dodecyl sulfate at elevated temperature allowing the solubilization of membr
## Abstract Electrophoretic techniques, and especially twoโdimensional gel electrophoresis (2โDE), have provided an indispensable set of tools for the separation of complex protein mixtures as well as for the identification of proteinโprotein interactions. Nevertheless, after its introduction more