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Optimized synthesis of radioiodinated rhodamine-123

✍ Scribed by Manhar M. Vora; Richard M. Lambrecht

Publisher
John Wiley and Sons
Year
1990
Tongue
French
Weight
447 KB
Volume
28
Category
Article
ISSN
0022-2135

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✦ Synopsis

Abstract

Rhodamine‐123 (Rh123) was iodinated with ^123^I in presence of Iodogen. Yield of the labelled Rh123 varied greatly depending upon various reaction parameters. Optimum reaction conditions with respect to molarity and pH of the buffer, reaction time, amounts of iodogen, carrier iodide and Rh123, were determined through carefully controlled reactions. Radiochemical yields of 50–60% were obtained within less than 2 hrs. using 0.05M NH~4~H~2~PO~4~ buffer at pH5, 200 μg iodogen, 250 μg Rh123 and no‐carrier‐added iodide. Separation of the labelled Rh123 in greater than 98% radiochemical purity was achieved by flash chromatography on silica gel. The overall isolated yield of radioiodinated Rh123 ranged from 25–35% with respect to the added radioiodide. TLC and HPLC methods were developed for analysis of the labelled products.

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