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Optimized synthesis of radioiodinated rhodamine-123

✍ Scribed by Manhar M. Vora; Richard M. Lambrecht

Publisher
John Wiley and Sons
Year
1989
Tongue
French
Weight
444 KB
Volume
27
Category
Article
ISSN
0022-2135

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✦ Synopsis

Rhodamine-123 (Rh123) w a s i o d i n a t e d w i t h l z 3 I i n presence of Iodogen. Y i e l d of t h e l a b e l l e d Rh123 v a r i e d g r e a t l y depending upon v a r i o u s r e a c t i o n parameters. Optimum r e a c t i o n c o n d i t i o n s w i t h r e s p e c t t o m o l a r i t y and pH of the b u f t e r , r e a c t i o n t i m e , amounts of iodogen, c a r r i e r i o d i d e and Kh123, were determined through c a r e f u l l y c o n t r o l l e d r e a c t i o n s . Radiochemical y i e l d s of 50-60% were o b t a i n e d w i t h i n less t h a n 2 h r s . u s i n g 0.05M NHqHzPO4 b u f f e r at pH5, 200 ug i o d o g e n , 250 v g Rh123 and n o -c a r r i e radded i o d i d e . S e p a r a t i o n of t h e l a b e l l e d Kh123 i n g r e a t e r t h a n 98% r a d i o c h e m i c a l p u r i t y was achieved by f l a s h chromatography on s i l i c a g e l . The o v e r a l l i s o l a t e d y i e l d of r a d i o i o d i n a t e d Rh123 ranged from 25-352 w i t h r e s p e c t t o t h e added r a d i o i o d i d e .

TLC and HPLC methods were developed f o r a n a l y s i s of t h e l a b e l l e d p r o d u c t s .

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