𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Optimization of peroxidase immobilization and of the design of packed-bed enzyme reactors for flow injection analysis

✍ Scribed by Bo Olsson; Lars Ögren

Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
894 KB
Volume
145
Category
Article
ISSN
0003-2670

No coin nor oath required. For personal study only.

✦ Synopsis

Packed-bed reactors containing horse radish peroxidase were optimized for use in flow injection systems. The most active and stable immobilizations were produced by ~ZO linkage to porous glass. The influence of pore and particle diameter as well as pH of immobilization, number of coupling sites, and enzyme purity were studied. The reactor behaviour could be accurately described by a theory derived on the assumptions of firstorder kinetics. The effects of internal and external mass transfer resistances were studied and the rate constants were evaluated. Design criteria for analytical reactors are discussed. A small particle diameter is shown to be of utmost importance in order to achieve low dispersion with fixed levels of back-pressure and conversion.

📜 SIMILAR VOLUMES

Amperometric flow-injection method for d
Amperometric flow-injection method for determination of biogenic diamines and hypoxanthine by combined use of immobilized enzyme reactors and a peroxidase electrode
✍ Toshio Yao; Masahiro Satomura; Tamotsu Wasa 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 430 KB

A flow-mJectlon system IS proposed for the determrnatlon of meat freshness, based on the simultaneous deternunation of polyammes and hypoxanthme A putrescme oxldase reactor and xanthme oxldase reactor were Incorporated at fured positrons m a flow system, which was based on the sphttmg of the flow af

Peroxidase- and Tetracyanoquinodimethane
Peroxidase- and Tetracyanoquinodimethane-Modified Graphite Paste Electrode for the Measurement of Glucose/Lactate/Glutamate Using Enzyme-Packed Bed Reactor
✍ P.C. Pandey; H.H. Weetall 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 466 KB

A flow injection analysis sensor for the measurement of glucose/lactate/glutamate is reported. The glucose oxidase/glutamate oxidase/lactate oxidase was immobilized on silanized controlled pore glass particles and packed into a Teflon column (i.d., \(1.2 \mathrm{~mm}\); length, 40 \(\mathrm{mm})\) t

Reactivation of an immobilized enzyme re
Reactivation of an immobilized enzyme reactor for the determination of acetylcholinesterase inhibitors Flow injection determination of paraoxon
✍ Cándido García de María; Teresa Manzano Muñoz; Alan Townshend 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 781 KB

A stopped-flow/flow-injection procedure is proposed for the spectrophotometric determination of paraoxon based on the inhibition of immobilized acetylcholinesterase. The irreversibly inhibited enzyme is reactivated with l,l'-trimethylene-bi&-formylpyridinium bromide) dioxime. The proposed procedure

Sequential determination of glucose, fru
Sequential determination of glucose, fructose and sucrose by flow-injection analysis with immobilized enzyme reactors and spectrophotometric detection
✍ Cándido García de María; Alan Townsend 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 640 KB

A flow-mjectlon spectrophotometrx procedure 1s described for the sequential deternunatlon of glucose, fructose and sucrose m a sample solution by usmg s&e and dual lmmobdtzed enzyme reactors m a smgle mamfold Concentrations III the range 10-5-10-3 M are determmed urlth analysis rates of 30-40 determ