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Reactivation of an immobilized enzyme reactor for the determination of acetylcholinesterase inhibitors Flow injection determination of paraoxon

✍ Scribed by Cándido García de María; Teresa Manzano Muñoz; Alan Townshend

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
781 KB
Volume
295
Category
Article
ISSN
0003-2670

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✦ Synopsis

A stopped-flow/flow-injection procedure is proposed for the spectrophotometric determination of paraoxon based on the inhibition of immobilized acetylcholinesterase.

The irreversibly inhibited enzyme is reactivated with l,l'-trimethylene-bi&-formylpyridinium bromide) dioxime. The proposed procedure has a detection limit of 4 x 10e9 M, a linear calibration range of 9 x 10e9-5 x lo-' M, a R.S.D. of 1.6% (n = 10) at 5.0 x lo-* M and a sample throughput frequency of 10 h-'. The enzyme reactor can be used for more than one thousand inhibition-reactivation cycles.

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