A spectrophotometric, continuous flow-injection, merging zones manifold including an enzyme reactor was used for the determination of magnesium ion in serum by exploiting the activating effect of MgfII) on the hydrolysis of o-nitrophenyl-B-o-galactopyranoside by j?-o-galactosidase immobiliied on con
Sequential determination of glucose, fructose and sucrose by flow-injection analysis with immobilized enzyme reactors and spectrophotometric detection
✍ Scribed by Cándido García de María; Alan Townsend
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 640 KB
- Volume
- 261
- Category
- Article
- ISSN
- 0003-2670
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✦ Synopsis
A flow-mjectlon spectrophotometrx procedure 1s described for the sequential deternunatlon of glucose, fructose and sucrose m a sample solution by usmg s&e and dual lmmobdtzed enzyme reactors m a smgle mamfold Concentrations III the range 10-5-10-3 M are determmed urlth analysis rates of 30-40 determmatlons h-' An R S D of 0 8% was obtamed for glucose and sucrose and of 1 1% for fructose (10 samples of 4 X 10m4 M m all cases)
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Immobdlzed sorbltol dehydrogenase was used as a reactor m a column hqmd chromatograplnc system for the selective detection of sucrose and fructose Sorbltol dehydrogenase was lmmoblhzed on controlled-pore glass The sugars were separated on a cation-exchange resm column with a mobde phase contammg 0 1
A flow injection method with chemiluminescence detection has been developed for the enzymatic determination of inorganic phosphate. Purine nucleoside phosphorylase, xanthine oxidase, and urate oxidase were immobilized on controlled-pore glass beads. Hydrogen peroxide released by the enzymatic reacti