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Column liquid chromatographic determination of sucrose and fructose with use of an enzyme reactor and spectrofluorimetric detection

✍ Scribed by Nobutoshi Kiba; Rumi Matsushita; Motohisa Furusawa

Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
332 KB
Volume
259
Category
Article
ISSN
0003-2670

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✦ Synopsis

Immobdlzed sorbltol dehydrogenase was used as a reactor m a column hqmd chromatograplnc system for the selective detection of sucrose and fructose Sorbltol dehydrogenase was lmmoblhzed on controlled-pore glass The sugars were separated on a cation-exchange resm column with a mobde phase contammg 0 1 M phosphate buffer (pH 7 0) and 100 PM NADH The disappearance of NASH was detected spectrofluorlmetncally For sucrose the linear workmg range was 50-500 PM, wth a detectlon hmlt of 10 PM (170 ng m a 5O+I InJectron), for fructose the range was 25-250 PM, with a detectlon hmlt of 5 pM (45 ng m a 50-~1 InJectIon)

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