Determination of phenols in environmentally relevant matrices with the use of liquid chromatography with an enzyme electrode detector

✍ Scribed by Kim R. Rogers; James Y. Becker; Joseph Wang; Fang Lu

• Publisher: John Wiley and Sons
• Year: 1999
• Weight: 107 KB
• Volume: 3
• Category: Article
• ISSN: 1086-900X
• DOI: 10.1002/(sici)1520-6521(1999)3:3<161::aid-fact3>3.0.co;2-x

✦ Synopsis

A simple and rapid assay using HPLC with a tyrosinase-containing carbon paste electrode (Tyr-CPE) detector is demonstrated for the detection of phenol, p-cresol, p-methoxyphenol, and p-chlorophenol in environmental matrices. These compounds were measured in contaminated aqueous soil leachate samples or acetone/acetonitrile extracts of soil and sludge samples. The dynamic range for this assay was 2-30 ppm for phenol and p-cresol. In addition to analysis by the Tyr-CPE detector held at a reducing potential (؊0.20 V), soil leachate and extracts were analyzed with the use of a carbon paste electrode (CPE) detector at oxidizing potentials (؉1.0 V or ؉1.2 V). Compared to the CPE, the Tyr-CPE detector was very stable, showing a signal loss of only 28% over 24 h of continuous operation. In addition, the signal from the CPE degraded rapidly by 65% over 10 sequential injections, whereas the signal of the Tyr-CPE decreased by only 5% for the same number of sequential injections. Because of the minimal sample preparation for analysis of soil leachate samples, the herein-reported chromatographic system with biosensor detector could be further developed to complement portable tyrosinase