Spectrophotometric determination of magnesium in serum by using a flow-injection system with an immobilized enzyme reactor

✍ Scribed by J.M. Fernández-Romero; M.D.Luque de Castro; M. Valcárcel; R. Quiles-Zafra

• Publisher: Elsevier Science
• Year: 1993
• Tongue: English
• Weight: 446 KB
• Volume: 283
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(93)85256-j

✦ Synopsis

A spectrophotometric, continuous flow-injection, merging zones manifold including an enzyme reactor was used for the determination of magnesium ion in serum by exploiting the activating effect of MgfII) on the hydrolysis of o-nitrophenyl-B-o-galactopyranoside by j?-o-galactosidase immobiliied on controlled-pore glass. The advantageous features of the proposed method (determination range 5-20 pmol l-l, within-run and between-run relative standard deviations of 0.78-2.91% and 1.7-3.21%, respectively) allow the determination of the analyte in serum samples with good recoveries (%-113%). The results obtained were consistent with those provided by the conventional atomic absorption spectrometric method.